FOXG1 Antibody (Center)

• Catalog Number: orb1927721
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB24681
• Tested applications: FC, IF, WB
• Predicted Reactivity: Drosophila, Other, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IF: 1:10~50, WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 52352 Da
• Target: This FOXG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-252 amino acids from the Central region of human FOXG1.
• UniProt ID: P55316
• NCBI: NP_005240.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Forkhead box protein G1, Brain factor 1, BF-1, BF1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FOXG1 Antibody (Center) in mouse brain tissue lysates (35 ug/lane). FOXG1 (arrow) was detected using the purified Pab.

FOXG1 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-FOXG1 Antibody (Center) at 1:1000 dilution + human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-FOXG1 Antibody (Center) at 1:1000 dilution. Lane 1: 293T/17 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 59kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of A549 cell stained with FOXG1 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.