FOXB1 Antibody

SKU: orb1247502

Description

FOXB1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 35.0kDa according to NP_036314.2. Recommended concentration: 1-3µg/mL.Immunocytochemsitry: Anonymous customer found nuclear staining at lumbar levels of the Mouse Spinal Cord at embryonic day 12.5. Recommended concentration 0.1-0.3µg/mL. Immunofluorescence: This antibody has been successfully used in IF for mouse brain at embryonic day 13.5.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-TSPASALHSVAVH
• Target: FOXB1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FOXB1, forkhead box B1, FKH5, HFKH-5

orb1247502 (1 ug/ml) staining of HepG2 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

FOXB1

UniProt

Q99853

RefSeq (Protein):NP_036314.2