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FOS/c-FOS Antibody

SKU: orb1536233

Description

FOS/c-FOS Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml)
ReactivityBovine, Canine, Equine, Guinea pig, Human, Monkey, Porcine, Sheep
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 37kD band observed in lysates of cell line HeLa (calculated MW of 40.7kD according to NP_005243.1). Recommended concentration: 0.3-1 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence C-SETARSVPDMDLS, from the internal region of the protein sequence according to NP_005243.1.
TargetFOS / c-FOS
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

FOS, Activator protein 1, AP-1, Cellular oncogene c-fos, Cellular oncogene fos, Proto-oncogene c-Fos, v-fos, C-FOS, G0s7, p55

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FOS/c-FOS Antibody

FOS antibody (0.3 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

FOS/c-FOS Antibody (orb1536233)

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50 μg
$ 640.00
DispatchUsually dispatched within 2-3 weeks
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