FLVCR Antibody

• Catalog Number: orb1247505
• Category: Antibodies
• Description: FLVCR Antibody
• Target: FLVCR1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-KTVMLSKQSESAI
• UniProt ID: Q9Y5Y0
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 59.9kDa according to NP_054772.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Anonymous customer reported uccessfully use in WB on cell line K562.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FLVCR1, feline leukemia virus subgroup C cellular
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_054772.1
• Expiration Date: 12 months from date of receipt.

orb1247505 (0.1 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.