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Catalog Number | orb1231295 |
---|---|
Category | Proteins |
Description | Flt-3 Ligand Recombinant Protein |
Target | Flt3l |
Tag | His Tag |
Form/Appearance | Lyophilized |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | > 90% as determined by SDS-PAGE. |
Protein Sequence | Gly 27 - Arg 188 |
UniProt ID | P49772 |
MW | 20.2 kDa |
Tested applications | ELISA, WB |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Source | HEK293 cells |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Alternative names | FLT3LG,FL,FLT3L,Flt3 ligand |
Note | For research use only |
NCBI | NP_038548.3 |
Mouse Flt-3 Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Flt-3, Fc Tag at 5 ug/ml (100 uL/well) can bind Mouse Flt-3 Ligand, His Tag with a linear range of 0.1-0.6 ng/mL.
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
19.9 kDa | |
Baculovirus |
> 95% as determined by SDS-PAGE | |
24-30 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
16.9 kDa | |
E.Coli |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
17.7 kDa | |
E.Coli |
> 85% by SDS-PAGE. | |
Recombinant Mouse Flt-3 Ligand Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Arg188) of mouse Flt-3 Ligand (Accession #NP_038548.3.) fused with an Fc, 6��His Tag at the C-terminus. |