FLOT2 Antibody

• Catalog Number: orb1256912
• Category: Antibodies
• Description: FLOT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 239-428 of human FLOT2 (NP_004466.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 47kDa
• Target: FLOT2
• UniProt ID: Q14254
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: FLOT2, flotillin 2, reggie-1, ESA, ESA1, M17S1, Fl
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using FLOT2 antibody (orb1256912) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of MCF7 cells using FLOT2 antibody (orb1256912). Blue: DAPI for nuclear staining.