Flotillin 2/FLOT2 Antibody

• Catalog Number: orb315131
• Category: Antibodies
• Description: Flotillin 2/FLOT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 47064 MW
• UniProt ID: Q14254
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Flotillin-2;Epidermal surface antigen;ESA;Membrane
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of RH35 cells using anti-Flotillin 2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U20S cells using anti-Flotillin 2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Flotillin 2 using anti-Flotillin 2 antibody.Lane 1:human 293T cell; 2:human HeLa cell; 3:human A375 cell; 4:human A549 cell; 5:rat thymus tissue; 6:rat lung tissue; 7:mouse thymus tissue; 8:mouse lung tissue.

IF analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in immunocytochemical section of A431 cells.

IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in a paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in a paraffin-embedded section of rat kidney tissue.

IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody.Flotillin 2 was detected in frozen section of rat kidney tissue.

IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody.Flotillin 2 was detected in frozen section of rat small intestine tissue.

IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody.Flotillin 2 was detected in frozen section of mouse kidney tissue.