FLOT1 Antibody

• Catalog Number: orb1257155
• Category: Antibodies
• Description: FLOT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 128-427 of human FLOT1 (NP_005794.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 47kDa
• Target: FLOT1
• UniProt ID: O75955
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: FLOT1, flotillin 1, reggie-2, integral membrane co
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using FLOT1 antibody (orb1257155) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of U2OS cells using FLOT1 antibody (orb1257155). Blue: DAPI for nuclear staining.