FLOT1 Antibody

• Catalog Number: orb1247981
• Category: Antibodies
• Description: FLOT1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SISQVNHKPLRTA
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in lysates of cell lines A431, Jurkat and K562 (calculated MW of 47.4kDa according to NP_005794.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FLOT1
• UniProt ID: O75955
• NCBI: NP_001305804.1, NP_005794.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FLOT1, flotillin 1, reggie-2, integral membrane co
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in lysates of cell lines A431, Jurkat and K562 (calculated MW of 47.4kDa according to NP_005794.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
• Expiration Date: 12 months from date of receipt.

orb1247981 (0.1 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247981 (3.8 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.