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Catalog Number | orb1239385 |
---|---|
Category | Antibodies |
Description | FLIP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP.The sequence is identical in all FLIP splice variants.The immunogen is located within the first 50 amino acids of FLIP. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 53 kDa Observed: 53 kDa |
Target | CFLAR |
UniProt ID | O15519 |
NCBI | AAC51622 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | FLIP Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Cas Read more... |
Note | For research use only |
Application notes | FLIP antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 µg/ml.
Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/mL.
Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 µg/mL.
Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 µg/mL.
Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 µg/ml. Red: FLIP Antibody (orb1239385) Blue: DAPI staining.
IF, IHC-Fr, IHC-P, WB | |
Equine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |