FLI1 monoclonal antibody (M05), clone 1F6

• Catalog Number: orb2292998
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant FLI1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1F6
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG2b Kappa
• Immunogen: FLI1 (AAH01670, 1 a.a. ~ 452 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MDGTIKEALSVVSDGQSLFDSAYGAAAHLPKADMAASGSPDYGQPHKINPLPPQQEWISQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERISKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY
• NCBI: AAH01670
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of monoclonal antibody to FLI1 on HeLa cell. [antibody concentration 10 ug/ml]

Western Blot analysis of FLI1 expression in transfected 293T cell line by FLI1 monoclonal antibody (M05), clone 1F6. Lane 1: FLI1 transfected lysate (50.982 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of FLI1 over-expressed 293 cell line, cotransfected with FLI1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FLI1 monoclonal antibody (M05), clone 1F6 (Cat # orb2292998). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (75.46 KDa).