CASP8AP2 Antibody

SKU: orb1239799

Description

CASP8AP2 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  FLASH antibody can be used for detection of FLASH by Western blot at 0.5 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid.The immunogen is located within the last 50 amino acids of FLASH.
• Target: CASP8AP2
• Molecular Weight: 220 kDa
• Purification: FLASH Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: FLASH Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FLASH Antibody: CED-4, FLASH, RIP25, KIAA1315, CASP8-associated protein 2, FLICE-associated huge protein

Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 µg/mL.

Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 µg/mL.

Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 µg/mL.

Quick Database Links

Gene Symbol

CASP8AP2

UniProt

Q9UKL3

RefSeq:AF165161