Flagellin TLR5 Mutant Recombinant Protein

SKU: orb1230104

Description

Flagellin TLR5 Mutant Recombinant Protein

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This recombinant proteins is for research use only.

Key Properties

• Source: E. coli
• Biological Activity: Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.
• Target: fliC
• Tag: His Tag
• Purity: >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
• Protein Sequence: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.

Storage & Handling

• Storage: Stable for at least 6 months after receipt when stored at -20°C.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Contains PBS. Reconstitute with 100 μl sterile water.
• Concentration: 0.1mg/ml after reconstitution.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FliC (TLR5 Mutant)

Quick Database Links

Gene Symbol

fliC

UniProt

P50591

RefSeq (Protein):NP_460912