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Description
Research Area
Immunology & Inflammation
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays. |
| Target | fliC |
| Tag | His Tag |
| Purity | >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag. |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. |
| Concentration | 0.1mg/ml after reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−FliC (TLR5 Mutant)
Quality Guarantee
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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