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Fibulin 5 Antibody

Catalog Number: orb1247508

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1247508
CategoryAntibodies
DescriptionFibulin 5 Antibody
TargetFBLN5
ClonalityPolyclonal
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Form/AppearanceLiquid
Concentration500 ug/mL
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ImmunogenThe immunogen for this antibody is: C-RPIKGPREIQLDLE
UniProt IDQ9UBX5
Tested applicationsELISA, WB
Application notesPeptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Colon, Heart and Ovary lysates (calculated MW of 50.2kDa according to NP_006320.2). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesFBLN5, fibulin 5 , ARMD3, DANCE, EVEC, FLJ90059, U
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NoteFor research use only
NCBINP_006320.2
Fibulin 5 Antibody

orb1247508 (0.1 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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