Fibronectin/Ugl-Y3 Rabbit Polyclonal Antibody

SKU: orb221410

Description

Fibronectin/Ugl-Y3 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Osteogenesis, Stem Cell Research

Images & Validation

• Tested Applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Fibronectin/Ugl-Y3 (2201-2300/2386aa)
• Target: FN1
• Molecular Weight: 259 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CIG; Cold insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; ED-B; fibronectin 1; FINC; FN 1; FN; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor; MSF; Transformation sensitive protein; FINC_HUMAN; Fibronectin; Cold-insoluble globulin; Ugl-Y3.

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-Fibronectin/Ugl-Y3 (orb221410) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 263 kD, Observed band size: 263 kD.

Tissue/Cell: HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Fibronectin/Ugl-Y3) Polyclonal Antibody, Unconjugated (orb221410) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

FN1

UniProt

P02751