Fibronectin/FN1 Rabbit Polyclonal Antibody

SKU: orb3117801

Description

The Fibronectin/FN1 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, and WB applications. This antibody targets the Fibronectin in Human samples.

Research Area

Cardiovascular Research; Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg /1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
• Reactivity: Human
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli-derived human Fibronectin/FN1 recombinant protein (Position: D869-W2014). Human FN1 shares 91.2% and 91.4% amino acid (aa) sequence identity with mouse and rat FN1, respectively.
• Target: Fibronectin
• Molecular Weight: 272 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CIG; Cold insoluble globulin; ED B; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF

Quick Database Links

Gene Symbol

Fibronectin

UniProt

P02751