Fibronectin antibody

• Catalog Number: orb27335
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Fibronectin
• Species/Host: Rabbit
• Tested applications: ELISA, IF, IHC-P, RIA
• Immunogen: Purified fibronectin from chicken plasma
• Concentration: app. 1 mg/ml
• Dilution range: (purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
• Conjugation: Unconjugated
• Hazard Information: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
• UniProt ID: P11722
• Storage: Storage: -20°C
• Buffer/Preservatives: Affinity purified antibody lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no rAlbumin and sodium azide! Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C. For further dilution use appropriate antibody diluent, e.g. Art. No. PU002 for IHC.
• Note: For research use only
• Application notes: Positive Control: Chicken skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of chicken skin using Fibronectin antibody