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Catalog Number | orb345615 |
---|---|
Category | Antibodies |
Description | Fibroblast Activation Protein antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein. |
Concentration | 1.1 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:20,000, WB: 1:500 to 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with FAP from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | P97321 |
NCBI | 6679749 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Fap antibody, Fibroblast activation pr Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3) using Fibroblast Activation Protein antibody
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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