Fibrillarin antibody

• Catalog Number: orb1140453
• Category: Antibodies
• Description: Mouse monoclonal antibody to Fibrillarin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 38F3
• Tested applications: ICC, WB
• Reactivity: Other
• Immunogen: Raised against yeast fibrillarin, a highly conserved nucleolar protein present in small nucleolar ribonucleoprotein (snoRNP) complexes.
• Concentration: 100ug/100ul
• Dilution range: For western blots of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods: 1:500 to 1:2,500. To detect mammalian fibrillarin on western blots by ECL: 1:250. For immunofluorescence on yeast cells: 1:500 to 1:2,500. For IF of mammalian cells: 1:250.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
• Note: For research use only
• Application notes: For western blots of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods: 1:500 to 1:2,500. To detect mammalian fibrillarin on western blots by ECL: 1:250. For immunofluorescence on yeast cells: 1:500 to 1:2,500. For IF of mammalian cells: 1:250.
• Expiration Date: 12 months from date of receipt.