Fibrillarin Antibody

• Catalog Number: orb1523984
• Category: Antibodies
• Description: Fibrillarin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 271 and 321
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Fibrillarin
• UniProt ID: P22087
• NCBI: NP_001427.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: RNA, U3 small nucleolar interacting protein 1; RNU
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse Fibrillarin by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (50µg) , Jurkat (50µg) , and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-Fibrillarin antibody orb1523984 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human Fibrillarin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Fibrillarin antibody orb1523984 used for IP at 6 µg/mg lysate.