FHL1 Antibody (C-term)

• Catalog Number: orb1929763
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This FHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-240 amino acids from the C-terminal region of human FHL1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q13642
• MW: 36263 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB19645
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Four and a half LIM domains protein 1, FHL-1, Skel
• Research Area: Cardiovascular Research
• Note: For research use only
• NCBI: NP_001153174.1, NP_001153173.1, NP_001153176.1, NP_001153175.1, NP_001153172.1, NP_001153171.1, NP_001440.2, NP_001161291.1
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of widr cells using FHL1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with FHL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of FHL1 Antibody (C-term) in NIH-3T3 cell line lysates (35 ug/lane). FHL1 (arrow) was detected using the purified Pab.

Western blot analysis of FHL1 (arrow) using rabbit polyclonal FHL1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FHL1 gene.