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Catalog Number | orb614114 |
---|---|
Category | Antibodies |
Description | FHL FHL1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human FHL1 recombinant protein (Position: A2-P323). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | "Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human" |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 32 kDa |
UniProt ID | Q13642 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Four and a half LIM domains protein 1; FHL-1; Skel Read more... |
Note | For research use only |
Application notes | "Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of PC-3 cells using anti-FHL antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.
IF analysis of FHL using anti-FHL antibody.
IHC analysis of FHL using anti-FHL antibody.
IHC analysis of FHL using anti-FHL antibody.
WB analysis using anti-FHL antibody.Lane 1:placenta tissue, Lane 2:HEK293 cell, Lane 3:PC-3 cell.
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