FH antibody

• Catalog Number: orb19547
• Category: Antibodies
• Description: Goat polyclonal antibody to FH
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.01-0.03 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 54.6
• Target: Fumarase / FH
• Entrez: 2271
• Protein Sequence: HPNDHVNKSQSSND
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti fumarate hydratase antibody, anti HLRCC antib
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Kidney lysate using FH antibody

Immunohistochemical staining of Human Skeletal Muscle using FH antibody

Immunohistochemical staining of Human Heart using FH antibody