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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Assay 1: The ED50 was determined by the dose - dependent stimulation of thymidine uptake by BaF3 cells expressing FGF - receptors is ≤ 0.5 ng/mL, corresponding to a specific activity of ≥ 2 x 106 units/mg. Assay 2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 10.0 ng/mL, corresponding to a specific activity of ≥ 1 x 105 units/mg. |
| Target | FGF17 |
| Purity | Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT |
Storage & Handling
−| Storage | The lyophilized FGF-17 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted FGF-17 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing FGF-17 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HH20, FGF-13, Fibroblast growth factor 17, FGF-17
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003858.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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