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Catalog Number | orb1247686 |
---|---|
Category | Antibodies |
Description | FCGR2B Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-PDALEEPDDQNRI |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell line Daudi (calculated MW of 34.0kDa according to NP_003992.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | FCGR2B |
UniProt ID | P31994 |
NCBI | NP_001002273.1, NP_001002275.1, NP_001177757.1, NP_001002274.1, NP_003992.3 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | FCGR2B, CD32, FCG2, FCGR2, IGFR2, Fc fragment of I Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell line Daudi (calculated MW of 34.0kDa according to NP_003992.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. |
Expiration Date | 12 months from date of receipt. |
orb1247686 (1 ug/ml) staining of Daudi cell lysate (RIPA buffer, (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247686 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
orb1247686 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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