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Catalog Number | orb19010 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to CD32b |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Reactivity | Human |
Dilution range | ELISA: 1:32000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 34.0; 31.9; 31.9; 34.0; 33.4 |
Target | CD32 / FCGR2B |
Entrez | 2213 |
Protein Sequence | PDALEEPDDQNRI |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti-CD32 antibody, anti- CD32 antigen antibody, a Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 34kDa band observed in Human myeloid erythroblast cell line K562 and Human monocytic cell line U937 lysates (calculated MW of 34.0kDa according to NP_003992.3;). In transfected HEK293 transiently expressing CD32 a band of approx. 40kDa (glycoprotein) is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of K562 lysate using CD32b antibody
Western blot analysis of HEK293 lysate using CD32b antibody
Immunohistochemical staining of Human Liver using CD32b antibody
Immunohistochemical staining of Human Placenta using CD32b antibody
ICC, IF, IHC-P, WB | |
Guinea pig, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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