FBW7 Antibody

• Catalog Number: orb1527913
• Category: Antibodies
• Description: FBW7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: FBW7
• UniProt ID: Q969H0
• NCBI: NP_361014.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; FBW6; SEL
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human FBW7 by western blot. Samples: Whole cell lysate (50 µg) from My-La CD4+, SU-DHL-1, VLN3G2, and NCI-H1975 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FBW7 antibody (orb1527913) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human FBW7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from VLN3G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FBW7 antibody (orb1527913) used for IP at 6 µg per reaction. FBW7 was also immunoprecipitated by a previous lot of this antibody (orb1527913) and a second antibody against a different epitope of FBW7.