FBW7 Antibody

• Catalog Number: orb1527910
• Category: Antibodies
• Description: FBW7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: FBW7
• UniProt ID: Q969H0
• NCBI: NP_361014.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; FBW6; SEL
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human FBW7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-FBW7 antibody orb1527910.

Detection of human FBW7 by immunohistochemistry.Sample: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-FBW7 (Cat. No. orb1527910) used at 1: 5, 000 (0.2μg/ml). Detection: DAB.