FBP1 Rabbit Polyclonal Antibody

SKU: orb5232

Description

FBP1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=2ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FBP1 (31-130/338aa)
• Target: FBP1
• Molecular Weight: 37 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

6-bisphosphatase 1; 6-bisphosphate 1-phosphohydrolase 1; D fructose 1 6 bisphosphate 1 phosphohydrolase 1; D-fructose-1; EC 3.1.3.11; F16P1_HUMAN; FBP; FBP 1; FBP1; FBPase 1; Fructose 1 6 bisphosphatase 1; Fructose bisphosphatase 1; Fructose-1; Growth inhibiting protein 17; Liver fructose bisphosphatase.

Blank control (black line): MCF-7. Primary Antibody (green line): Rabbit Anti-FBP1 antibody (orb5232), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (FBP1) polyclonal Antibody, Unconjugated (orb5232) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

FBP1

UniProt

P09467