Fatty Acid Synthase Antibody, KO Validated

• Catalog Number: orb1274398
• Category: Antibodies
• Description: Fatty Acid Synthase Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (NP_004095.4).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 275kDa
• Target: FASN
• UniProt ID: P49327
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Fatty acid synthase, [Acyl-carrier-protein] S-acet
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of H460 cells, using Fatty Acid Synthase antibody at 1:200 dilution.

Western blot analysis of extracts from normal (control) and Fatty Acid Synthase knockout (KO) 293T cells, using Fatty Acid Synthase antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human colon using Fatty Acid Synthase antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using Fatty Acid Synthase antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using Fatty Acid Synthase antibody. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of H460 cells, using 3 ug Fatty Acid Synthase antibody. Western blot was performed from the immunoprecipitate using Fatty Acid Synthase antibody at a dilution of 1:1000.