FASN monoclonal antibody (M01), clone 3F2-1F3

• Catalog Number: orb2293095
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant FASN.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3F2-1F3
• Tested applications: ELISA, IF, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: FASN (AAH07909, 1 a.a. ~ 439 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASGLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG
• NCBI: AAH07909
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged FASN is 0.1 ng/ml as a capture antibody.

FASN monoclonal antibody (M01), clone 3F2-1F3 Western Blot analysis of FASN expression in 293.

Immunofluorescence of monoclonal antibody to FASN on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to FASN on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 10 ug/ml]

Immunoprecipitation of FASN transfected lysate using anti-FASN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FASN MaxPab rabbit polyclonal antibody.

Western Blot analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M01), clone 3F2-1F3. Lane 1: FASN transfected lysate (48.3 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (74.03 KDa).