FasL Antibody / Fas Ligand

• Catalog Number: orb2635376
• Category: Antibodies
• Description: Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an importantcomponent of specific effector mechanisms in immuno-surveillance againstvirus-infected or transformed cells. Two mechanisms appear to account forthis activity, one of which is the perforin-based process. Independently, aFAS-based mechanism involves the transducing molecule FAS (also designated Apo-1) and its ligand (FAS-L). The human FAS protein is a cell surfaceglycoprotein that belongs to a family of receptors that includes CD40, nervegrowth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of whichundergo apoptosis in response to treatment with antibody to FAS. Thesefindings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FASmay be critically involved in CTL-mediated cytotoxicity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FASLG/4453
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portin of amino acids 107-222 from the human protein was used as the immunogen for the FasL antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This FasL antibody is available for research use only.
• UniProt ID: P48023
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the FasL antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with FasL antibody (clone FASLG/4453). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free FasL antibody (clone FASLG/4453) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using FasL antibody (clone FASLG/4453). These results demonstrate the foremost specificity of the FASLG/4453 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.