Fas Ligand Rabbit Polyclonal Antibody (FITC)

SKU: orb15600

Description

Fas Ligand Rabbit Polyclonal Antibody (FITC)

Images & Validation

• Tested Applications: FC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Fas Ligand (196-281/281aa)
• Target: FASLG
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Tumor necrosis factor ligand superfamily member 6; FASLG; APO1L; Apoptosis(APO 1) antigen ligand 1; Fas-L; Apoptosis antigen ligand 1; APTL; CD178; CD-178; CD 178; CD95L; CD95 ligand; CD95-L; CD95L protein; FAS antigen ligand; Fas L; FASL; Fas ligand(TNF superfamily member 6); Generalized lymphoproliferative disease; TNF superfamily member 6; TNFSF6; TNFL6_HUMAN; Apoptosis antigen ligand; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form; FASLG.

Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-Fas Ligand antibody (orb15600), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

FASLG