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Catalog Number | orb613937 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to Fas |
Species/Host | Human |
Clonality | Monoclonal |
Clone Number | R-125224 |
Tested applications | ELISA, FC |
Reactivity | Human |
Isotype | Human IgG |
Immunogen | R-125224 is generated by the humanization of the murine HFE7A anti-Fas antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas or the L5178YA1 cell line expressing murine Fas, but not to the parental WR19L or L5178Y cells. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | Fas |
UniProt ID | P25445 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | CD95; h-HFE7A; Tumor necrosis factor receptor supe Read more... |
Note | For research use only |
Application notes | R-125224 shows the same binding affinity and the same ability to induce apoptosis in WR19L12a cells that express human Fas as the parental murine HFE7A antibody. R-125224 selectively induces apoptosis in type I activated lymphocytes but not in type II cells. R-125224 is able to induce apoptosis in the human lymphoid cell lines H9 and SKW6.4, as well as activated human lymphocytes, when cross-linked with anti-hIgG secondary antibodies. The antibody is unable to induce apoptosis in HPB-ALL cells, Jurkat cells or human hepatocytes. R-125224 has been used in vivo where it has been shown to greatly reduce the number of activated human human CD3+ Fas+ T cells in a SCID mouse model possessing a functional human immune system. Fas antigen tissue distribution in cynomolgus monkeys with collagen-induced arthritis at the arm joint (CIA monkeys) has been studied using [125I]-Labeled R-125224. High radioactivity in the bone marrow, thymus, lungs, liver, adrenals, spleen, ovaries, axillary lymph node and mesenteric lymph node compared to the radioactivity in the plasma was observed, which correlates with Fas expression. Fas can also be detected by R-125224 by ELISA. |
Expiration Date | 12 months from date of receipt. |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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