Daxx Antibody

• Catalog Number: orb308845
• Category: Antibodies
• Description: Daxx Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Daxx recombinant protein (Position: K56-R345). Human Daxx shares 88.3% and 88.6% amino acid (aa) sequence identity with mouse and rat Daxx, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry , 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 81373 MW
• UniProt ID: Q9UER7
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Death domain-associated protein 6;Daxx;hDaxx;ETS1-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of 293T cells using anti-Daxx antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Daxx using anti-Daxx antibody.Lane 1:human K562 cell; 2:rat PC-12 cell; 3:mouse thymus tissue.

IF analysis of Daxx using anti-Daxx antibody. Daxx was detected in immunocytochemical section of U20S cells.

IF analysis of Daxx using anti-Daxx antibody and anti-Tubulin Alpha antibody.Daxx was detected in immunocytochemical section of HeLa cell.

IHC analysis of DAXX using anti-DAXX antibody. DAXX was detected in a paraffin-embedded section of rat intestine tissue.

IHC analysis of DAXX using anti-DAXX antibody. DAXX was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of DAXX using anti-DAXX antibody.DAXX was detected in immunocytochemical section of SMMC-7721 Cell.

IHC analysis of DAXX using anti-DAXX antibody.DAXX was detected in immunocytochemical section of A549 Cell.