FARP1 Antibody

• Catalog Number: orb1247684
• Category: Antibodies
• Description: FARP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: GEIEQRPTPGSRL-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments showed an approx 110-120kDa band in Human Frontal Cortex lysates (calculated MW of 119kDa according to NP_ 005757.1).. An additional band was also consistently observed at 40kDa and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FARP1
• UniProt ID: Q9Y4F1
• NCBI: NP_001273768.1, NP_001001715.2, NP_005757.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FARP1, FERM, RhoGEF (ARHGEF) and pleckstrin domain
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments showed an approx 110-120kDa band in Human Frontal Cortex lysates (calculated MW of 119kDa according to NP_ 005757.1).. An additional band was also consistently observed at 40kDa and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247684 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

orb1247684 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1247684 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.