FAM73A Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb187931
• Category: Antibodies
• Description: FAM73A Rabbit Polyclonal Antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF
• Predicted Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FAM73A (101-200/632aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IF=1:100-500, ICC/IF=1:100-500
• Conjugation: FITC
• Target: MIGA1
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: DKFZp686M07166; FA73A_HUMAN; fam73a; Family with s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (Black line): U-87MG (Black). Primary Antibody (green line): Rabbit Anti-FAM73A antibody (orb187931), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.