Factor XIII-A antibody

• Catalog Number: orb21227
• Category: Antibodies
• Description: Precipitating polyclonal rabbit antiserum to human coagulation Factor XIII-A. Plasma FXIII is an active transglutaminase until it is activated (FXIIIa). It consists of two A subunits joined as a dimer, connected to two B subunits (A2B2). Each A subunit has a molecular weight of 75,000 and each B subunit of 80,000, making an aggregate molecular weight of the whole molecule of 320,000. Normal plasma contains about 20mg/ml. Platelet FXIII consists of only A subunits synthesized in the megakaryocytes. It accounts for about 50% of the total blood FXIII activity with a linear relationship between FXIII level and platelet counts. Factor XIII-A reacts with both plasma and platelet FXIII. Antiserum raised against the B subunit (RAHu/FXIII-S) reacts exclusively with native and activated FXIIIa in plasma. FXIIIa results from activation of FXIII by thrombin after the release of fibrinopeptides A from fibrinogen. Highly purified FXIII-A is used for the immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP
• Reactivity: Human
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• UniProt ID: P00488
• Source: Plasma FXIII is an active transglutaminase until it is activated (FXIIIa). It consists of two A subunits joined as a dimer, connected to two B subunits (A2B2). Each A subunit has a molecular weight of 75,000 and each B subunit of 80,000, making an aggregate molecular weight of the whole molecule of 320,000. Normal plasma contains about 20mg/ml. Platelet FXIII consists of only A subunits synthesized in the megakaryocytes. It accounts for about 50% of the total blood FXIII activity with a linear relationship between FXIII level and platelet counts. This product reacts with both plasma and platelet FXIII. Antiserum raised against the B subunit reacts exclusively with native and activated FXIIIa in plasma. FXIIIa results from activation of FXIII by thrombin after the release of fibrinopeptides A from fibrinogen. Highly purified FXIII-A is used for the immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Storage: Storage: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day
• Note: For research use only
• Expiration Date: 12 months from date of receipt.