F13A1 Antibody

• Catalog Number: orb1252150
• Category: Antibodies
• Description: F13A1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: F13A1/1448
• Tested applications: ELISA, FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b, kappa
• Immunogen: A human partial recombinant protein corresponding to amino acids 46-181 was used as the immunogen for the Factor XIIIa antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: 2-4 ug/ml; order the BSA-free formulation for coatingImmunofluorescence: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the Factor XIIIa antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: F13A1
• UniProt ID: P00488
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: F13A1, coagulation factor XIII, A1 polypeptide, RP
• Note: For research use only
• Application notes: ELISA: 2-4 ug/ml; order the BSA-free formulation for coatingImmunofluorescence: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the Factor XIIIa antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using Factor XIIIa antibody (clone F13A1/1448). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human histiocytoma with Factor XIIIa antibody (clone F13A1/1448). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of 1) partial human recombinant protein and 2) human HeLa lysate with Factor XIIIa antibody (clone F13A1/1448). Expected molecular weight ~83 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Factor XIIIa Antibody (clone F13A1/1448). Confirmation of Integrity and Purity of the Antibody.