Factor VIIIa light chain Rabbit Polyclonal Antibody

• Catalog Number: orb156822
• Category: Antibodies
• Description: Factor VIIIa light chain Rabbit Polyclonal Antibody. FVIII is a large molecule glycoprotein composed of 2,332 amino acids and is divided into multiple functional domains (arranged in the order of A1-A2-B-A3-C1-C2).
• Target: F8
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Factor VIIIa light chain (1751-1850/2351aa)
• UniProt ID: P00451
• MW: 75 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: coagulation factor VIII; Ahf; Antihemophilic facto
• Note: For research use only

Sample: Lymph node (Mouse) Lysate at 40 ug, Testis (Mouse) Lysate at 40 ug, Primary: Anti-Factor VIIIa light chain (orb156822) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 75 kD, Observed band size: 75 kD.

Sample: Raji (human) cell Lysate at 30 ug, Primary: Anti-Factor VIIIa light chain (orb156822) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 75kD, Observed band size: 85kD.