F9 Antibody

SKU: orb1259341

Description

F9 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-192 of human Factor IX / F9 (NP_000124.1).
• Target: F9
• Molecular Weight: Observed: 50kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, Coagulation factor IXa light chain, Coagulation factor IXa heavy chain, F9

Western blot analysis of extracts of various cell lines, using Factor IX/F9 antibody (orb1259341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of rat liver using Factor IX/F9 Polyclonal Antibody (orb1259341) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of human placenta using Factor IX/F9 Polyclonal Antibody (orb1259341) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

F9

UniProt

P00740