Factor D Rabbit Polyclonal Antibody

• Catalog Number: orb156818
• Category: Antibodies
• Description: Factor D Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human Factor D/Adipsin (21-120/253aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 24 kDa
• Target: CFD
• UniProt ID: P00746
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: adipsin; Adipsin/complement factor D; adn; C3 conv
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Protein: A549 (human) cell lysates at 40 ug, spleen disease in mouse at 40 ug, Primary: Anti-Factor D (orb156818) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 24 kD, Observed band size: 28/75 kD.

Tissue/Cell: Human lung cancer tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Factor D Polyclonal Antibody, Unconjugated (orb156818) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.