Fab Biotin Antibody

SKU: orb345931

Description

Fab antibody

Images & Validation

• Tested Applications: DOT, ELISA
• Dilution Range: ELISA: 1:5,000
• Application Notes:
  Fab Anti-Biotin Antibody has been tested by ELISA, dot blot, and SDS-PAGE and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG Fab
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Purity: Fab fragment of Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-papain or anti-Goat IgG F(c).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat Fab Anti-Biotin Antibody, Goat Fab Fragment Anti-Biotin Antibody

Dot Blot results of Goat Fab Anti-Biotin Antibody. Dots are top row (T) Biotin-BSA conjugated or bottom row (B) BSA alone: at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Goat Fab Anti-Biotin at 1 µg/ml for 1hr at RT. Secondary Antibody: Donkey Anti-Goat HRP (p/n orb347032) at 1:40000 for 30 min at RT.

SDS-PAGE results of Goat Fab Anti-Biotin Antibody. Lane 1: reduced Goat Fab Anti-Biotin. Lane 2: Opal PreStained Molecular Weight Ladder. Lane 3: non-reduced Goat Fab Anti-Biotin. Load: 1 µg. 4-20% Gel, Coomassie Blue Stained.