FA83D Rabbit Polyclonal Antibody

SKU: orb183501

Description

FA83D Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Canine, Equine, Human, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FA83D (151-225/225aa)
• Target: FAM83D
• Molecular Weight: 64 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

2310007D09Rik; BB104611; C20orf129; CHICA; dJ616B8.3; FA83D_HUMAN; FAM83D; Family with sequence similarity 83, member D; FLJ38341; MGC92947; Protein FAM83D; RGD1565583; RP23-119H5.1; Spindle protein CHICA.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FA83D) Polyclonal Antibody, Unconjugated (orb183501) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

FAM83D

UniProt

Q9H4H8

References

Xiaodong Yu, Yaxun Li, Fanshuang Kong, Qun Xu METTL3 regulates FAM83D m6A modification to accelerate tumorigenesis of triple-negative breast cancer via the Wnt/β-catenin pathway Toxicol In Vitro, 105746 (2023)

PubMed: 38043628

Applications

WB

Reactivity

Human

Jinjin Li 1, Jianbing Tian 1, Ming Ma 2, Zhiruo Qin 1, Bingji Cao 3, Jiangshuo Yang 4, Xuexiao Wang 5, Xingxiao Yang FAM83D promotes the progression of 4NQO-induced esophageal carcinoma via inhibiting FBWX7 Exp Cell Res, (2024)

PubMed: 39260674

Applications

IHC

Reactivity

Human