FA83D Rabbit Polyclonal Antibody

• Catalog Number: orb183501
• Category: Antibodies
• Description: FA83D Rabbit Polyclonal Antibody
• Target: FAM83D
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Canine, Equine, Human, Mouse, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human FA83D (151-225/225aa)
• UniProt ID: Q9H4H8
• MW: 64 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 2310007D09Rik; BB104611; C20orf129; CHICA; dJ616B8
• Research Area: Cancer Research, Cell Cycle, Scaffold Proteins, Si
• Note: For research use only

References

Xiaodong Yu, Yaxun Li, Fanshuang Kong, Qun Xu METTL3 regulates FAM83D m6A modification to accelerate tumorigenesis of triple-negative breast cancer via the Wnt/β-catenin pathway Toxicol In Vitro, 105746 (2023)

PubMed: 38043628

Applications

WB

Reactivity

Human

Jinjin Li 1, Jianbing Tian 1, Ming Ma 2, Zhiruo Qin 1, Bingji Cao 3, Jiangshuo Yang 4, Xuexiao Wang 5, Xingxiao Yang FAM83D promotes the progression of 4NQO-induced esophageal carcinoma via inhibiting FBWX7 Exp Cell Res, (2024)

PubMed: 39260674

Applications

IHC

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FA83D) Polyclonal Antibody, Unconjugated (orb183501) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.