Ezrin (Phospho-Tyr353) Rabbit Polyclonal Antibody

SKU: orb14792

Description

This is a rabbit polyclonal antibody targeting Ezrin. It is unconjugated and suitable for use in IHC, WB applications. The antibody is reactive with human samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500~1:1000; IHC: 1:50~1:100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN
• Target: Ezrin
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cytovillin; EZRI; VIL2; Villin 2; p81

Quick Database Links

Gene Symbol

Ezrin

UniProt

P15311

Juliana Martins da Costa-Pessoa 1, Mariana Charleaux de Ponte 2, Heitor Macedo Braz 1, Mário Costa Cruz 3, Guilherme Lopes Gonçalves 1, Maria Oliveira-Souza 1 Renal ischemia/reperfusion injury triggers proximal tubular apoptosis and NHE3 dysfunction via p38MAPK/ezrin signaling pathway Am J Physiol Renal Physiol, (2025)

PubMed: 40610014