EZH2 antibody

• Catalog Number: orb378050
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EZH2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human EZH2
• Dilution range: WB: 1:500-2000, IF/ICC: 1:20-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EZH2
• Entrez: 2146, 14056
• UniProt ID: Q61188, Q15910
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: KMT6; Histone-lysine N-methyltransferase EZH2; ENX
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EZH2 expression in HL60 (A), SW620 (B), MCF7 (C), mouse ovary (D) whole cell lysates. (Predicted band size: 79; 81; 84; 85; 86 kD; Observed band size: 98 kD)

Immunohistochemical analysis of EZH2 staining in human thyroid cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of EZH2 from 0.5 mg Hela whole cell extract lysate, using Anti-EZH2 Antibody.