EYA4 Rabbit Polyclonal Antibody

SKU: orb544595

Description

EYA4 Rabbit Polyclonal Antibody

Research Area

DNA and RNA Research, Neuroscience, Transcription Factors, Visual System

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EYA4 (131-230/639aa)
• Target: EYA4
• Molecular Weight: 70 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMD1J; Deafness, autosomal dominant 10; DFNA 10; DFNA10; dJ78N10.1(eyes absent(Drosophila) homolog 4); dJ78N10.1(eyes absent); EYA 4; eya4; EYA4_HUMAN; Eyes absent 4; Eyes absent homolog 4(Drosophila); Eyes absent homolog 4; HGNC:3522; OTTHUMP00000040267.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EYA4) Polyclonal Antibody, Unconjugated (orb544595) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

EYA4

UniProt

O95677