ETS1 Rabbit Polyclonal Antibody

• Catalog Number: orb10622
• Category: Antibodies
• Description: ETS1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Guinea pig, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Ets1 (321-441/441aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: ETS1
• UniProt ID: P14921
• RRID: AB_10767601
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Avian erythroblastosis virus E26(v ets) oncogene h
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-ETS1 antibody (orb10622) Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Thymus tissue lysates, Lane 3: Mouse Lung tissue lysates, Lane 4: Rat Lymph node tissue lysates, Lane 5: Human Jurkat cell lysates, Lane 6: Human U251 cell lysates, Primary: Anti-ETS1 (orb10622) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kDa, Observed band size: 50 kDa.

Sample: Spleen (Mouse) Lysate at 30 ug, Primary: Anti-ETS1 (orb10622) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.

Tissue/Cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ETS1 Polyclonal Antibody, Unconjugated (orb10622) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.