Estrogen Receptor beta Antibody

• Catalog Number: orb749491
• Category: Antibodies
• Description: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A C-terminus fragment of recombinant human ER-b protein was used as the immunogen for the Estrogen Receptor beta antibody.
• UniProt ID: Q92731
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Estrogen Receptor beta antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: ESR2/686
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the Estrogen Receptor beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This Estrogen Receptor beta antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Estrogen Receptor beta antibody (ESR2/686).

IHC: Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Estrogen Receptor beta antibody (ESR2/686).

Flow cytometry testing of BT474 cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Estrogen Receptor beta antibody (ESR2/686).

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with Estrogen Receptor beta antibody (ESR2/686).

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Estrogen Receptor beta antibody (ESR2/686).

Flow cytometry testing of MCF-7 cells. Black: cells alone; Green: isotype control; Red: PE-labeled Estrogen Receptor beta antibody (ESR2/686).

SDS-PAGE Analysis of Purified, BSA-Free Estrogen Receptor beta Antibody (clone ESR2/686). Confirmation of Integrity and Purity of the Antibody.