ESR1 Antibody

• Catalog Number: orb1252770
• Category: Antibodies
• Description: ESR1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ER506
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIHC (Formalin-paraffin): 1-2 ug/ml (1)Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.1. FFPE testing requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min and cooling at RT for 20 minutes.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ESR1
• UniProt ID: P03372
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, E
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIHC (Formalin-paraffin): 1-2 ug/ml (1)Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.1. FFPE testing requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min and cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

IHC testing of human breast invasive ductal carcinoma with ER antibody (ER506).

Intracellular FACS staining of MCF-7 cells using ER antibody (red) and isotype control (green).

FACS testing of human MCF-7: Black = cells alone; Green = isotype control; Red = Estrogen Receptor PE conjugate.

Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~66kDa.